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KMID : 1191520100150010047
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Journal of Korean Traditional Oncology
2010 Volume.15 No. 1 p.47 ~ p.61
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Effect of Allergen Removed Rhus Verniciflua Extract on Inhibition of Tumor Metastasis
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Park Jae-hyun
Moon Goo
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Abstract
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Objective: The aim of this present study is to evaluate the inhibitory effect of allergen removed Rhus verniciflua (ARV) on Matrix Metalloproteinase-9 (MMP-9), Matrix Metalloproteinase-2 (MMP-2) which is considered to have a clinically important role in tumor metastasis.
Methods: The inhibitory effects of standardized extract of ARV on the MMP-2, MMP-9 were investigated by spectrofluorometer while the inhibitory effects on the active MMP-2, pro MMP-2, pro MMP-9 were investigated by zymography. Antimetastatic effect of standardized extract of ARV was investigated in vitro on human fibrosarcoma cell (HT1080)’s invasion through Matrigel.
Results: The standardized extract of ARV showed inhibitory effects on the active MMP-2 (IC50, /ml), active MMP-9 (IC50, /ml) depending on concentrations which was determined by spectrofluorometer. The standardized extract of ARV showed inhibitory effects on the active MMP-2, pro MMP-2, pro MMP-9 depending on concentrations which was determined by zymography. However its inhibitory effect on pro MMP-9 was relatively weaker rather than active MMP-2, pro MMP-2. The standardized extract of ARV showed inhibitory effects in vitro on human fibrosarcoma cell (HT1080)’s invasion through Matrigel according to concentration.
Conclusions: These results indicate that standardized extract of ARV has antimetastatic effect through inhibit again MMP-2, MMP-9. Also its inhibitory effect is more powerful on active MMP-2, pro MMP-2 than on active MMP-9, pro MMP-9. It is necessary to conduct further studies on other MMP families, TIMP, and each component of standardized extract of ARV.
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KEYWORD
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MMP-2, MMP-9, Rhus Verniciflua, metastasis, invasion, cancer
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